Recombinant:Article Title: The spatiotemporal dynamics of microglia across the human lifespan.
Article Snippet: .. Antibodies Rabbit anti-IBA1 Wako Chemicals Cat# 019-19741 Goat anti-IBA1 Abcam Cat# ab5076 Mouse anti-IBA1 Abcam Cat# ab283319 Mouse anti-MHC-II/HLA-DP/DQ/DR Dako, Agilent Cat# M077501-2 Rabbit anti-PU.1 Cell Signalling Cat# 2258S Biotinylated anti-RCA1 Vector Labs Cat# B10855 Rabbit anti-TMEM119 Abcam Cat# ab185333 Mouse anti-CD68 Dako, Agilent Cat# GA609 Mouse anti-SOX2 Santa Cruz Cat# sc365823 Mouse anti-Ki67 Dako, Agilent Cat# M724029-2 Rabbit anti-Caspase3 (cleaved) Cell Signalling Cat# 9664S Goat anti-rabbit Alexafluor 488 Invitrogen Cat# A32731 Goat anti-mouse Alexafluor 568 Invitrogen Cat# A11031 Horse anti-rabbit HRP (DAB, brown) Vector Labs Cat# SK4103 Horse anti-mouse HRP (DAB, brown) Vector Labs Cat# MP7724 Horse anti-rabbit HRP (DAB + Nickel, black) Vector Labs Cat# SK100 Horse anti-rabbit AP (magenta) Vector Labs Cat# MP7724 Horse anti-rabbit AP (blue) Vector Labs Cat# SK5400 Biological samples Human post-mortem developmental tissues Wellcome/MRC HDBR N/A Human post-mortem developmental tissues Zagreb Brain Collection N/A Human post-mortem developmental tissues Thomas Willis Oxford Brain Bank N/A Human post-mortem developmental tissues Southampton Brain Bank N/A Human post-mortem postnatal tissues Southampton Brain Bank N/A Human post-mortem postnatal tissues Edinburgh Brain Bank N/A Human post-mortem postnatal tissues Cambridge Brain Bank N/A Human post-mortem postnatal tissues Southwest Dementia Brain Bank N/A Chemicals, peptides, and recombinant proteins Immpress Duet Kit Vector Labs Cat# MP7724 ImmPACT DAB-EqV substrate kit Vector Labs Cat# SK4103 BCIP/NBT substrate kit Vector Labs Cat# SK5400 Vectastain Elite ABC kit Vector Labs Cat# PK6100 Haematoxylin QS Vector Labs Cat# H3404-100 Methyl Green Vector Labs Cat# H3042 Dual enzyme block Dako, Agilent Cat# S2003 Periodic Acid Sigma-Aldrich (Merck) Cat# P7875 Schiff’s Reagent Sigma-Aldrich (Merck) Cat# 3952016 Alcian blue Sigma-Aldrich (Merck) Cat# A5268 Cresyl Violet acetate Sigma-Aldrich (Merck) Cat# C5042 Citric acid Sigma-Aldrich (Merck) Cat# 251275 Sodium borohydride Sigma-Aldrich (Merck) Cat# 213462 Vectashield antifade mounting medium Vector Labs Cat #H1000-10 DPX mounting medium Sigma-Aldrich (Merck) Cat # 06522 Trueblack autofluorescence quencher Biotium Cat# 23007 (Continued on next page) e1 Developmental Cell 57, 2127–2139.e1–e6, September 12, 2022 .. Deposited data Bulk human RNAseq data This paper Gerrelli et al., 2015 & Lindsay et al., 2016 Human adult microglial juvenile RNAseq data This paper Galatro et al., 2017 Human adult microglia adult RNAseq data This paper Gosselin et al., 2017 Human development single-cell RNA seq This paper Bian et al., 2020 Human development single-cell RNA seq This paper Kracht et al., 2020 Human development single-cell RNA seq This paper Fan et al., 2020 Human development single-cell RNA seq This paper Cao et al., 2020 Software and algorithms Prism 9 Graphpad https://www.graphpad.com/ scientific- software/prism/ Fiji Schindelin et al., 2012 https://imagej.net/software/fiji/ R studio R Studio Development Team https://www.rstudio.com/ QGIS (v2.18.3) QGIS Development Team https://www.qgis.org/en/site/ Seurat (v3.2.2) Stuart et al., 2019 https://satijalab.org/seurat/ Fluoview Olympus Olympus https://www.olympus-lifescience.com/en/laser- scanning/fv3000/ Imagescope Aperio (v12.4.3) Leica biosystems https://www.leicabiosystems.com/en-gb/ digital-pathology/manage/aperio-imagescope/ VS110 Desktop (v4) Olympus https://www.olympus-lifescience.com/en/ solutions-based-systems/vs200/ NDP.view2 Hamamatsu Photonics https://www.hamamatsu.com/jp/en/product/ life-science-and-medical-systems/digitalslide-scanner.html Gimp (v2.10.22) Gimp Development Team https://docs.gimp.org/2.10/en/ Samtools (v1.1) Li et al., 2009 http://samtools.sourceforge.net/ EdgeR Robinson et al., 2010; Soneson and Robinson, 2018 https://bioconductor.org/packages/release/ bioc/html/edgeR.html HTSeq (v0.6.1) Anders et al., 2015 https://academic.oup.com/bioinformatics/ article/31/2/166/2366196 Trinity (v2.4.0) Haas et al., 2013 https://www.nature.com/articles/nprot.2013.084 Metascape Metascape Development Team https://metascape.org/gp/index.html#/main/step1 STAR v2.5.2b Dobin et al., 2013 https://academic.oup.com/bioinformatics/article/ 29/1/15/272537?login=false
Article Title: The spatiotemporal dynamics of microglia across the human lifespan
Article Snippet: .. Antibodies Rabbit anti-IBA1 Wako Chemicals Cat# 019-19741 Goat anti-IBA1 Abcam Cat# ab5076 Mouse anti-IBA1 Abcam Cat# ab283319 Mouse anti-MHC-II/HLA-DP/DQ/DR Dako, Agilent Cat# M077501-2 Rabbit anti-PU.1 Cell Signalling Cat# 2258S Biotinylated anti-RCA1 Vector Labs Cat# B10855 Rabbit anti-TMEM119 Abcam Cat# ab185333 Mouse anti-CD68 Dako, Agilent Cat# GA609 Mouse anti-SOX2 Santa Cruz Cat# sc365823 Mouse anti-Ki67 Dako, Agilent Cat# M724029-2 Rabbit anti-Caspase3 (cleaved) Cell Signalling Cat# 9664S Goat anti-rabbit Alexafluor 488 Invitrogen Cat# A32731 Goat anti-mouse Alexafluor 568 Invitrogen Cat# A11031 Horse anti-rabbit HRP (DAB, brown) Vector Labs Cat# SK4103 Horse anti-mouse HRP (DAB, brown) Vector Labs Cat# MP7724 Horse anti-rabbit HRP (DAB + Nickel, black) Vector Labs Cat# SK100 Horse anti-rabbit AP (magenta) Vector Labs Cat# MP7724 Horse anti-rabbit AP (blue) Vector Labs Cat# SK5400 Biological samples Human post-mortem developmental tissues Wellcome/MRC HDBR N/A Human post-mortem developmental tissues Zagreb Brain Collection N/A Human post-mortem developmental tissues Thomas Willis Oxford Brain Bank N/A Human post-mortem developmental tissues Southampton Brain Bank N/A Human post-mortem postnatal tissues Southampton Brain Bank N/A Human post-mortem postnatal tissues Edinburgh Brain Bank N/A Human post-mortem postnatal tissues Cambridge Brain Bank N/A Human post-mortem postnatal tissues Southwest Dementia Brain Bank N/A Chemicals, peptides, and recombinant proteins Immpress Duet Kit Vector Labs Cat# MP7724 ImmPACT DAB-EqV substrate kit Vector Labs Cat# SK4103 BCIP/NBT substrate kit Vector Labs Cat# SK5400 Vectastain Elite ABC kit Vector Labs Cat# PK6100 Haematoxylin QS Vector Labs Cat# H3404-100 Methyl Green Vector Labs Cat# H3042 Dual enzyme block Dako, Agilent Cat# S2003 Periodic Acid Sigma-Aldrich (Merck) Cat# P7875 Schiff’s Reagent Sigma-Aldrich (Merck) Cat# 3952016 Alcian blue Sigma-Aldrich (Merck) Cat# A5268 Cresyl Violet acetate Sigma-Aldrich (Merck) Cat# C5042 Citric acid Sigma-Aldrich (Merck) Cat# 251275 Sodium borohydride Sigma-Aldrich (Merck) Cat# 213462 Vectashield antifade mounting medium Vector Labs Cat #H1000-10 DPX mounting medium Sigma-Aldrich (Merck) Cat # 06522 Trueblack autofluorescence quencher Biotium Cat# 23007 (Continued on next page) e1 Developmental Cell 57, 2127–2139.e1–e6, September 12, 2022 .. Deposited data Bulk human RNAseq data This paper Gerrelli et al., 2015 & Lindsay et al., 2016 Human adult microglial juvenile RNAseq data This paper Galatro et al., 2017 Human adult microglia adult RNAseq data This paper Gosselin et al., 2017 Human development single-cell RNA seq This paper Bian et al., 2020 Human development single-cell RNA seq This paper Kracht et al., 2020 Human development single-cell RNA seq This paper Fan et al., 2020 Human development single-cell RNA seq This paper Cao et al., 2020 Software and algorithms Prism 9 Graphpad https://www.graphpad.com/ scientific- software/prism/ Fiji Schindelin et al., 2012 https://imagej.net/software/fiji/ R studio R Studio Development Team https://www.rstudio.com/ QGIS (v2.18.3) QGIS Development Team https://www.qgis.org/en/site/ Seurat (v3.2.2) Stuart et al., 2019 https://satijalab.org/seurat/ Fluoview Olympus Olympus https://www.olympus-lifescience.com/en/laser- scanning/fv3000/ Imagescope Aperio (v12.4.3) Leica biosystems https://www.leicabiosystems.com/en-gb/ digital-pathology/manage/aperio-imagescope/ VS110 Desktop (v4) Olympus https://www.olympus-lifescience.com/en/ solutions-based-systems/vs200/ NDP.view2 Hamamatsu Photonics https://www.hamamatsu.com/jp/en/product/ life-science-and-medical-systems/digitalslide-scanner.html Gimp (v2.10.22) Gimp Development Team https://docs.gimp.org/2.10/en/ Samtools (v1.1) Li et al., 2009 http://samtools.sourceforge.net/ EdgeR Robinson et al., 2010; Soneson and Robinson, 2018 https://bioconductor.org/packages/release/ bioc/html/edgeR.html HTSeq (v0.6.1) Anders et al., 2015 https://academic.oup.com/bioinformatics/ article/31/2/166/2366196 Trinity (v2.4.0) Haas et al., 2013 https://www.nature.com/articles/nprot.2013.084 Metascape Metascape Development Team https://metascape.org/gp/index.html#/main/step1 STAR v2.5.2b Dobin et al., 2013 https://academic.oup.com/bioinformatics/article/ 29/1/15/272537?login=false ll OPEN ACCESSResource
Blocking Assay:Article Title: The spatiotemporal dynamics of microglia across the human lifespan.
Article Snippet: .. Antibodies Rabbit anti-IBA1 Wako Chemicals Cat# 019-19741 Goat anti-IBA1 Abcam Cat# ab5076 Mouse anti-IBA1 Abcam Cat# ab283319 Mouse anti-MHC-II/HLA-DP/DQ/DR Dako, Agilent Cat# M077501-2 Rabbit anti-PU.1 Cell Signalling Cat# 2258S Biotinylated anti-RCA1 Vector Labs Cat# B10855 Rabbit anti-TMEM119 Abcam Cat# ab185333 Mouse anti-CD68 Dako, Agilent Cat# GA609 Mouse anti-SOX2 Santa Cruz Cat# sc365823 Mouse anti-Ki67 Dako, Agilent Cat# M724029-2 Rabbit anti-Caspase3 (cleaved) Cell Signalling Cat# 9664S Goat anti-rabbit Alexafluor 488 Invitrogen Cat# A32731 Goat anti-mouse Alexafluor 568 Invitrogen Cat# A11031 Horse anti-rabbit HRP (DAB, brown) Vector Labs Cat# SK4103 Horse anti-mouse HRP (DAB, brown) Vector Labs Cat# MP7724 Horse anti-rabbit HRP (DAB + Nickel, black) Vector Labs Cat# SK100 Horse anti-rabbit AP (magenta) Vector Labs Cat# MP7724 Horse anti-rabbit AP (blue) Vector Labs Cat# SK5400 Biological samples Human post-mortem developmental tissues Wellcome/MRC HDBR N/A Human post-mortem developmental tissues Zagreb Brain Collection N/A Human post-mortem developmental tissues Thomas Willis Oxford Brain Bank N/A Human post-mortem developmental tissues Southampton Brain Bank N/A Human post-mortem postnatal tissues Southampton Brain Bank N/A Human post-mortem postnatal tissues Edinburgh Brain Bank N/A Human post-mortem postnatal tissues Cambridge Brain Bank N/A Human post-mortem postnatal tissues Southwest Dementia Brain Bank N/A Chemicals, peptides, and recombinant proteins Immpress Duet Kit Vector Labs Cat# MP7724 ImmPACT DAB-EqV substrate kit Vector Labs Cat# SK4103 BCIP/NBT substrate kit Vector Labs Cat# SK5400 Vectastain Elite ABC kit Vector Labs Cat# PK6100 Haematoxylin QS Vector Labs Cat# H3404-100 Methyl Green Vector Labs Cat# H3042 Dual enzyme block Dako, Agilent Cat# S2003 Periodic Acid Sigma-Aldrich (Merck) Cat# P7875 Schiff’s Reagent Sigma-Aldrich (Merck) Cat# 3952016 Alcian blue Sigma-Aldrich (Merck) Cat# A5268 Cresyl Violet acetate Sigma-Aldrich (Merck) Cat# C5042 Citric acid Sigma-Aldrich (Merck) Cat# 251275 Sodium borohydride Sigma-Aldrich (Merck) Cat# 213462 Vectashield antifade mounting medium Vector Labs Cat #H1000-10 DPX mounting medium Sigma-Aldrich (Merck) Cat # 06522 Trueblack autofluorescence quencher Biotium Cat# 23007 (Continued on next page) e1 Developmental Cell 57, 2127–2139.e1–e6, September 12, 2022 .. Deposited data Bulk human RNAseq data This paper Gerrelli et al., 2015 & Lindsay et al., 2016 Human adult microglial juvenile RNAseq data This paper Galatro et al., 2017 Human adult microglia adult RNAseq data This paper Gosselin et al., 2017 Human development single-cell RNA seq This paper Bian et al., 2020 Human development single-cell RNA seq This paper Kracht et al., 2020 Human development single-cell RNA seq This paper Fan et al., 2020 Human development single-cell RNA seq This paper Cao et al., 2020 Software and algorithms Prism 9 Graphpad https://www.graphpad.com/ scientific- software/prism/ Fiji Schindelin et al., 2012 https://imagej.net/software/fiji/ R studio R Studio Development Team https://www.rstudio.com/ QGIS (v2.18.3) QGIS Development Team https://www.qgis.org/en/site/ Seurat (v3.2.2) Stuart et al., 2019 https://satijalab.org/seurat/ Fluoview Olympus Olympus https://www.olympus-lifescience.com/en/laser- scanning/fv3000/ Imagescope Aperio (v12.4.3) Leica biosystems https://www.leicabiosystems.com/en-gb/ digital-pathology/manage/aperio-imagescope/ VS110 Desktop (v4) Olympus https://www.olympus-lifescience.com/en/ solutions-based-systems/vs200/ NDP.view2 Hamamatsu Photonics https://www.hamamatsu.com/jp/en/product/ life-science-and-medical-systems/digitalslide-scanner.html Gimp (v2.10.22) Gimp Development Team https://docs.gimp.org/2.10/en/ Samtools (v1.1) Li et al., 2009 http://samtools.sourceforge.net/ EdgeR Robinson et al., 2010; Soneson and Robinson, 2018 https://bioconductor.org/packages/release/ bioc/html/edgeR.html HTSeq (v0.6.1) Anders et al., 2015 https://academic.oup.com/bioinformatics/ article/31/2/166/2366196 Trinity (v2.4.0) Haas et al., 2013 https://www.nature.com/articles/nprot.2013.084 Metascape Metascape Development Team https://metascape.org/gp/index.html#/main/step1 STAR v2.5.2b Dobin et al., 2013 https://academic.oup.com/bioinformatics/article/ 29/1/15/272537?login=false
Article Title: The spatiotemporal dynamics of microglia across the human lifespan
Article Snippet: .. Antibodies Rabbit anti-IBA1 Wako Chemicals Cat# 019-19741 Goat anti-IBA1 Abcam Cat# ab5076 Mouse anti-IBA1 Abcam Cat# ab283319 Mouse anti-MHC-II/HLA-DP/DQ/DR Dako, Agilent Cat# M077501-2 Rabbit anti-PU.1 Cell Signalling Cat# 2258S Biotinylated anti-RCA1 Vector Labs Cat# B10855 Rabbit anti-TMEM119 Abcam Cat# ab185333 Mouse anti-CD68 Dako, Agilent Cat# GA609 Mouse anti-SOX2 Santa Cruz Cat# sc365823 Mouse anti-Ki67 Dako, Agilent Cat# M724029-2 Rabbit anti-Caspase3 (cleaved) Cell Signalling Cat# 9664S Goat anti-rabbit Alexafluor 488 Invitrogen Cat# A32731 Goat anti-mouse Alexafluor 568 Invitrogen Cat# A11031 Horse anti-rabbit HRP (DAB, brown) Vector Labs Cat# SK4103 Horse anti-mouse HRP (DAB, brown) Vector Labs Cat# MP7724 Horse anti-rabbit HRP (DAB + Nickel, black) Vector Labs Cat# SK100 Horse anti-rabbit AP (magenta) Vector Labs Cat# MP7724 Horse anti-rabbit AP (blue) Vector Labs Cat# SK5400 Biological samples Human post-mortem developmental tissues Wellcome/MRC HDBR N/A Human post-mortem developmental tissues Zagreb Brain Collection N/A Human post-mortem developmental tissues Thomas Willis Oxford Brain Bank N/A Human post-mortem developmental tissues Southampton Brain Bank N/A Human post-mortem postnatal tissues Southampton Brain Bank N/A Human post-mortem postnatal tissues Edinburgh Brain Bank N/A Human post-mortem postnatal tissues Cambridge Brain Bank N/A Human post-mortem postnatal tissues Southwest Dementia Brain Bank N/A Chemicals, peptides, and recombinant proteins Immpress Duet Kit Vector Labs Cat# MP7724 ImmPACT DAB-EqV substrate kit Vector Labs Cat# SK4103 BCIP/NBT substrate kit Vector Labs Cat# SK5400 Vectastain Elite ABC kit Vector Labs Cat# PK6100 Haematoxylin QS Vector Labs Cat# H3404-100 Methyl Green Vector Labs Cat# H3042 Dual enzyme block Dako, Agilent Cat# S2003 Periodic Acid Sigma-Aldrich (Merck) Cat# P7875 Schiff’s Reagent Sigma-Aldrich (Merck) Cat# 3952016 Alcian blue Sigma-Aldrich (Merck) Cat# A5268 Cresyl Violet acetate Sigma-Aldrich (Merck) Cat# C5042 Citric acid Sigma-Aldrich (Merck) Cat# 251275 Sodium borohydride Sigma-Aldrich (Merck) Cat# 213462 Vectashield antifade mounting medium Vector Labs Cat #H1000-10 DPX mounting medium Sigma-Aldrich (Merck) Cat # 06522 Trueblack autofluorescence quencher Biotium Cat# 23007 (Continued on next page) e1 Developmental Cell 57, 2127–2139.e1–e6, September 12, 2022 .. Deposited data Bulk human RNAseq data This paper Gerrelli et al., 2015 & Lindsay et al., 2016 Human adult microglial juvenile RNAseq data This paper Galatro et al., 2017 Human adult microglia adult RNAseq data This paper Gosselin et al., 2017 Human development single-cell RNA seq This paper Bian et al., 2020 Human development single-cell RNA seq This paper Kracht et al., 2020 Human development single-cell RNA seq This paper Fan et al., 2020 Human development single-cell RNA seq This paper Cao et al., 2020 Software and algorithms Prism 9 Graphpad https://www.graphpad.com/ scientific- software/prism/ Fiji Schindelin et al., 2012 https://imagej.net/software/fiji/ R studio R Studio Development Team https://www.rstudio.com/ QGIS (v2.18.3) QGIS Development Team https://www.qgis.org/en/site/ Seurat (v3.2.2) Stuart et al., 2019 https://satijalab.org/seurat/ Fluoview Olympus Olympus https://www.olympus-lifescience.com/en/laser- scanning/fv3000/ Imagescope Aperio (v12.4.3) Leica biosystems https://www.leicabiosystems.com/en-gb/ digital-pathology/manage/aperio-imagescope/ VS110 Desktop (v4) Olympus https://www.olympus-lifescience.com/en/ solutions-based-systems/vs200/ NDP.view2 Hamamatsu Photonics https://www.hamamatsu.com/jp/en/product/ life-science-and-medical-systems/digitalslide-scanner.html Gimp (v2.10.22) Gimp Development Team https://docs.gimp.org/2.10/en/ Samtools (v1.1) Li et al., 2009 http://samtools.sourceforge.net/ EdgeR Robinson et al., 2010; Soneson and Robinson, 2018 https://bioconductor.org/packages/release/ bioc/html/edgeR.html HTSeq (v0.6.1) Anders et al., 2015 https://academic.oup.com/bioinformatics/ article/31/2/166/2366196 Trinity (v2.4.0) Haas et al., 2013 https://www.nature.com/articles/nprot.2013.084 Metascape Metascape Development Team https://metascape.org/gp/index.html#/main/step1 STAR v2.5.2b Dobin et al., 2013 https://academic.oup.com/bioinformatics/article/ 29/1/15/272537?login=false ll OPEN ACCESSResource
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